A “green” solvent-free industrial process (patent pending) is here described for a grape seed extract (GSE) preparation (Ecovitis™) obtained from selected seeds of Veneto region wineries, in the northeast of Italy, by water and selective tangential flow filtration at different porosity.

Phytochemistry Methods Specification
Proanthocyanidins Folin Ciocalteau (calculated on dry substance) accuracy ±15% ≥ 95 %
Oligomeric proanthocyanidins Bate Smith (calculated on dry substance) accuracy ± 10% ≥ 95 %
Oligomeric proanthocyanidins Gpc Contents (calculated on dry substance) ≥ 85 %
Gallic Acid HPLC (calculated on dry substance) ≤ 0,5 %
Monomers (epicatechin + catechin) HPLC (calculated on dry substance) ≤ 5,0 %
Dimers (Pro B1 + Pro B2) HPLC (calculated on dry substance) ≤ 5,0 %
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